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A dominant-negative G s (DNG s ) with 9 mutations (S54N, G226A, E268A, N271K, K274D, R280K, T284D, I285T and A366S) were generated by site-directed mutagenesis to stabilize interactions with the subunits in the GIPRG s complex 34

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As chronic heart failure creates a substrate for AF (24,25), the beneficial effects of exendin-4 on heart failure-induced atrial arrhythmias may be partly responsible for the improvements in cardiac function elicited by the drug